Wednesday, February 22, 2012

Measure and compare the size of the kill ...

Prepared Petri dish should be kept in strattera 40mg the refrigerator to use and always stored upside down (ie media in upper dish, cover the bottom). This allows the condensation that forms in the lid from falling on the violation and bacteria growing surface. Prepare sterilized water with boiling water and let cool to room temperature. When everything is ready for use, let the Petri dish to room temperature before sampling (about one hour). Prepare antiseptic drives using a paper puncher to drive with a piece of filter paper or paper towel. Soak one disc each antibacterial agent for testing. Set aside to step 6. Collect bacteria from each location using one swab for each new location. Fill a small test tube partly full sterilized water. Dip bacteria laden swab in the water. This will transfer some bacteria collected in the water. Now, to instill in the Petri dish filling water so that the entire surface is covered. Pour the excess water. Repeat for each sample by bacteria, fresh water and clean test tube each time. Place the pre-antiseptic disks in each Petri vaccinated. Place lid on dish, tape closed, store upside down. Be sure to mark each Petri dish with a name or number. Let rise in a warm quiet place, best in the environment of about 100 F (37 ° C) - not in the sun or heating register. You should see growth within a few days. You should also see a halo around each disk indicates no growth area. Measure and compare the size of the zone of injury to determine the effectiveness of each antibacterial agent. Before disposing of dishes in the trash the bacteria should be destroyed. Pour a small amount of bleach over the colonies while holding dish in the sink. Warning - do not allow bleach to touch the skin, eyes or clothing. It will burn! .

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